Wednesday, February 27, 2013

Experimental Methods

A. Application of the Ion Samples to the Paper

a)Get a capillary tube, a small beaker of pee, and chromatography typography. usance the tube to get some water and practice using aliquots to the cover. Apply metal cations samples to chromatography piece of music.
b)Place paper towels on bench. Use a pair of gloves to get a piece of chromatography paper. Place chromatography paper on towels.
c)Make sure as shooting paper is 18cm wide and 12cm high.
d)Obtain a clean ruler. neb and draw pencil line 1 cm from straightest limit and this will be origin. From left side of paper euphony 1.20cm to the right. Repeat this process 13 times.
e)Label for each one distinguish mark in Figure 1. Note the order of cations, know mixture and unknow solutions.
f)Obtain five nitrate salts, each salt has different metal cation AgNO3, CO(NO3)2, Cu(NO3)3, Fe(NO3)2, and Ni(NO3)2 , a tube of cognize mixture that contains these cations and one unknown mixture. Record number of unknown.
g)Use capillary tube to create sample sides of 2-mm diameter. tactile sensation each known, individual metal cation twice, and dry the spots. Spot the known mixture four times. Spot the unknown sample collar times, drying the spot between each application and dry the spot between each application.

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h)Use an 800 mL beaker and a bed sheet of aluminum and fold to go the opening with 2-3 inches to spare. Take beaker, counterpoint and graduated cylinder to the top containing the eluting solvent. Measure 30 mL of eluting solvent into the graduated cylinder. Transfer solution into the large beaker and cover with aluminum. Put beaker on lab bench for 75 minutes.
i) displume paper into a cylinder with writing outside. With tape reassure paper into a cylinder shape. Make sure paper is not overlapping or makeing.

B) Elution of the Spotted Samples

a)Place the chromatography paper into 800 mL beaker containing the solution. Make sure paper does not touch sides of beaker. Recover the beaker with aluminum foil.
b)Let the beaker sit for 75 minutes. Use...If you insufficiency to get a full essay, order it on our website: Orderessay



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